Dmitrii Kriukov, Ekaterina Kuzmina, Evgeniy Efimov, Dmitry V. Dylov, Ekaterina E. Khrameeva

A critique of the epigenetic aging clocks for their high uncertainty in rejuvenation predictions during cellular reprogramming, due to misrepresentation in training data and inconsistent outcomes. It suggests incorporating uncertainty estimates into aging clock models for more accurate assessments.

Francesco Neri, Selma N. Takajjart, Chad A. Lerner, Pierre-Yves Desprez, Birgit Schilling, Judith Campisi, Akos A. Gerencser

The Fully-Automated Senescence Test (FAST) is an automated, image-based method for quantifying senescence in cultured cells by assessing key markers. It standardizes senescence measurement, supports various cell types and microscopes, and aids in evaluating compounds affecting senescence.

Eitan Moses, Tehila Atlan, Xue Sun, Roman Franek, Atif Siddiqui, Georgi K. Marinov, Sagiv Shifman, David M. Zucker, Adi Oron-Gottesman, William J. Greenleaf, Ehud Cohen, Oren Ram, Itamar Harel

Germline manipulation in turquoise killifish shows sex-specific aging effects: depletion boosts female repair and male lifespan, indicating metabolic rejuvenation and pro-longevity genes, challenging classical evolutionary tradeoffs.

Steve Horvath, Joshua Zhang, Amin Haghani, Ake T. Lu, Zhe Fei

Methylation change rates in chromatin state and lifespan show an inverse relationship in a study of 125 mammalian species.

Andrei E. Tarkhov, Thomas Lindstrom-Vautrin, Sirui Zhang, Kejun Ying, Mahdi Moqri, Bohan Zhang, Alexander Tyshkovskiy, Orr Levy, Vadim N. Gladyshev

Epigenetic aging involves both co-regulated and stochastic DNA methylation changes, comparing tissue and single-cell data with development stages and RNA analysis. A new algorithm identifies CpG clusters, offering insights into aging interventions.